The DeepChek®-TB RpoB/InhA Genotyping Assay (K-18-RPOBINHA) is intended to determine drug resistance to isoniazid and rifampicin through PCR/sequencing.
DNA Sequencing • Polymerase Chain Reaction (PCR)
CHARACTERISTICS AND PERFORMANCES
Compatibility with Next Generation Sequencing platforms
Validated on any capillary electrophoresis instrument and on MiSeq, MiniSeq, (iSeq-100 ongoing) Ion PGM, Ion S5
RUO – ISO-9001 manufacturing
Types of samples
Decontaminated and concentrated sputum sediments
PCR reagents (enzymes, master mixes, primers, dNTPs...), protocols for Sanger & NGS sequencing, Sanger & NGS software analyses for 24 samples via a Cloud access
From sample to result in ~30 hours for NGS (depending on the platform)
inhA: codons 1 – 130 rpoB: codons 92 – 522
Compatible extraction methods
Automatic (MagNA Pure Compact Nucleic Acid Isolation Kit I), Manual (Manual extractions using QIAamp® Viral DNA - Qiagen)...
Data analysis and interpretation software
Amino-acid mutations detection, nucleotide changes detection, drug resistance, NGS-run quality report, clinical genotyping report...
Isoniazid and rifampicin based on different up-to-date guidelines
Unlimited updates & upgrades of the software, support, training...
Local servers, historical data import, integration with LIS and HIS, integration with sequencers, automation of the IT workflow, customization...
EXAMPLES OF REPORTS
DeepChek®-TB RpoB/InhA Genotyping Assay