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DeepChek®-TB RpoB/InhA Genotyping Assay

K-18-RPOBINHA

Description

The DeepChek®-TB RpoB/InhA Genotyping Assay (K-18-RPOBINHA) is intended to determine drug resistance to isoniazid and rifampicin through PCR/sequencing.

Methodology

DNA Sequencing • Polymerase Chain Reaction (PCR)

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More information on the DeepChek® Software – Click here

CHARACTERISTICS AND PERFORMANCES

Features

Compatibility with Next Generation Sequencing platforms

Any

Intended use

RUO – ISO-9001 manufacturing

Types of samples

Decontaminated and concentrated sputum sediments

Format

24 samples/kit

Content

PCR reagents (enzymes, master mixes, primers, dNTPs...), protocols for Sanger & NGS sequencing, Sanger & NGS software analyses for 24 samples via a Cloud access

Sensitivity

Study ongoing

Specificity

High

Reproducibility

>99%

Workflow

From sample to result in ~30 hours for NGS (depending on the platform)

Covered positions

inhA: codons 1 – 130 rpoB: codons 92 – 522

Compatible extraction methods

Automatic (MagNA Pure Compact Nucleic Acid Isolation Kit I), Manual (Manual extractions using QIAamp® Viral DNA - Qiagen)...

Data analysis and interpretation software

BacterioChek-TB

Available analyses

Amino-acid mutations detection, nucleotide changes detection, drug resistance, NGS-run quality report, clinical genotyping report...

Drug resistance

Isoniazid and rifampicin based on different up-to-date guidelines

Included services

Unlimited updates & upgrades of the software, support, training...

Options

Local servers, historical data import, integration with LIS and HIS, integration with sequencers, automation of the IT workflow, customization...

EXAMPLES OF REPORTS

 

Ordering information

Product

Reference

DeepChek®-TB RpoB/InhA Genotyping Assay

K-18-RPOBINHA