Description
The DeepChek®-TB RpoB/InhA Genotyping Assay (K-18-RPOBINHA) is intended to determine drug resistance to isoniazid and rifampicin through PCR/sequencing.
Methodology
DNA Sequencing • Polymerase Chain Reaction (PCR)
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CHARACTERISTICS AND PERFORMANCES
Features
Compatibility with Next Generation Sequencing platforms
Validated on any capillary electrophoresis instrument and on MiSeq, MiniSeq, (iSeq-100 ongoing) Ion PGM, Ion S5
Intended use
RUO – ISO-9001 manufacturing
Types of samples
Decontaminated and concentrated sputum sediments
Format
24 samples/kit
Content
PCR reagents (enzymes, master mixes, primers, dNTPs...), protocols for Sanger & NGS sequencing, Sanger & NGS software analyses for 24 samples via a Cloud access
Sensitivity
Study ongoing
Specificity
High
Reproducibility
>99%
Workflow
From sample to result in ~30 hours for NGS (depending on the platform)
Covered positions
inhA: codons 1 – 130 rpoB: codons 92 – 522
Compatible extraction methods
Automatic (MagNA Pure Compact Nucleic Acid Isolation Kit I), Manual (Manual extractions using QIAamp® Viral DNA - Qiagen)...
Data analysis and interpretation software
BacterioChek-TB
Available analyses
Amino-acid mutations detection, nucleotide changes detection, drug resistance, NGS-run quality report, clinical genotyping report...
Drug resistance
Isoniazid and rifampicin based on different up-to-date guidelines
Included services
Unlimited updates & upgrades of the software, support, training...
Options
Local servers, historical data import, integration with LIS and HIS, integration with sequencers, automation of the IT workflow, customization...
EXAMPLES OF REPORTS
Ordering information
Product
Reference
DeepChek®-TB RpoB/InhA Genotyping Assay
K-18-RPOBINHA
