Description
The UltraGene-HBV Assay is intended to be used for Viral Hepatitis B (HBV) viral load detection above 15 UI/mL through qPCR. When results are positive, it can also be used for genotyping and provides antiviral susceptibility information for reverse transcriptase inhibitors. It includes target-specific qPCR reagents compatible with either Sanger or Next Generation Sequencing platforms.
Methodology
DNA Sequencing • Polymerase Chain Reaction (PCR)
More information on the UltraGene Assays – Click here
More information on the DeepChek® Software – Click here
Characteristics and performances
Features
Compatibility with Light Cycler platforms
LightCycler® 480 System
Compatibility with Next Generation Sequencing platforms
Validated on MiSeq, MiniSeq, Ion PGM, Ion S5
Intended use
RUO – ISO-9001 manufacturing
Types of samples
Serum, plasma, DBS
Format
48 samples/kit or 96 samples/kit
Content
qPCR reagents (enzymes, master mixes, primers, dNTPs...), SANGER sequencing primers, protocols for SANGER and NGS sequencing
Sensitivity
15 UI/mL for 400 µL plasma/serum
Specificity
Validated on every genotype
Reproducibility
>99%
Workflow
From sample to result in less than 2 hours. Genotyping performed in ~15 hours for SANGER and ~30 hours for NGS (depending on the platform)
Covered positions
reverse transcriptase region of the polymerase gene
Compatible extraction methods
Automatic (MagNA Pure Compact Nucleic Acid Isolation Kit I - Roche, Promega - Abbott), Manual (Manual extractions using QIAamp® Viral RNA - Qiagen)...
Data analysis and interpretation software
Not included
Examples of reports
Viral Load Detection
Ordering information
Product
Reference
UltraGene-HBV Viral Load QUAL Assay (up to 24 samples)
K-17-UHBV24
UltraGene-HBV Viral Load QUAL Assay (up to 96 samples)
K-17-UHBV96
UltraGene-HBV Viral Load QUANT Assay (up to 24 samples)
K-17-UQTHBV24
UltraGene-HBV Viral Load QUANT Assay (up to 96 samples)
K-17-UQTHBV96
Downloads
General documentation
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General brochure
MSDS
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International
