UltraGene-HBV Assay

Description

The UltraGene-HBV Assay is intended to be used for Viral Hepatitis B (HBV) viral load detection above 15 UI/mL through qPCR. When results are positive, it can also be used for genotyping and provides antiviral susceptibility information for reverse transcriptase inhibitors. It includes target-specific qPCR reagents compatible with either Sanger or Next Generation Sequencing platforms.

Methodology

DNA Sequencing • Polymerase Chain Reaction (PCR)

More information on the UltraGene Assays – Click here
More information on the DeepChek® Software – Click here

Characteristics and performances

Features

Compatibility with Light Cycler platforms

LightCycler® 480 System

Compatibility with Next Generation Sequencing platforms

Validated on MiSeq, MiniSeq, Ion PGM, Ion S5

Intended use

RUO – ISO-9001 manufacturing

Types of samples

Serum, plasma, DBS

Format

48 samples/kit or 96 samples/kit

Content

qPCR reagents (enzymes, master mixes, primers, dNTPs...), SANGER sequencing primers, protocols for SANGER and NGS sequencing

Sensitivity

15 UI/mL for 400 µL plasma/serum

Specificity

Validated on every genotype

Reproducibility

>99%

Workflow

From sample to result in less than 2 hours. Genotyping performed in ~15 hours for SANGER and ~30 hours for NGS (depending on the platform)

Covered positions

reverse transcriptase region of the polymerase gene

Compatible extraction methods

Automatic (MagNA Pure Compact Nucleic Acid Isolation Kit I - Roche, Promega - Abbott), Manual (Manual extractions using QIAamp® Viral RNA - Qiagen)...

Data analysis and interpretation software

Not included

Examples of reports

Viral Load Detection

 

 

Ordering information

Product

Reference

UltraGene-HBV Viral Load QUAL Assay (up to 24 samples)

K-17-UHBV24

UltraGene-HBV Viral Load QUAL Assay (up to 96 samples)

K-17-UHBV96

UltraGene-HBV Viral Load QUANT Assay (up to 24 samples)

K-17-UQTHBV24

UltraGene-HBV Viral Load QUANT Assay (up to 96 samples)

K-17-UQTHBV96

Downloads

General documentation

  • General brochure

    EN

MSDS

  • International

    EN