Description
The DeepChek®Assay -HSV TK/POL (RUO) is intended to determine the presence of mutations within UL23 (thymidine kinase) and UL30 (DNA polymerase) conferring drug resistance to antiviral drugs through PCR/sequencing (NGS).
Methodology
DNA Sequencing • Polymerase Chain Reaction (PCR)
More information on the DeepChek® Assays – Click here
More information on the DeepChek® Software – Click here
CHARACTERISTICS AND PERFORMANCES
Features
Compatibility with Next Generation Sequencing platforms
Any
Intended use
RUO – ISO 13485 manufacturing
Types of samples
Serum, plasma and whole blood
Format
24 samples/kit
Content
PCR reagents (enzymes, master mixes, primers, dNTPs...), protocols for NGS sequencing, NGS software analyses for 24 samples via a Cloud access
Sensitivity
1250 UI/mL for 1mL plasma/serum. Protocols for low viral loads (>200 - 300 UI/mL) available
Specificity
High
Reproducibility
>99%
Workflow
From sample to result in ~30 hours for NGS (depending on the platform)
Covered positions
UL23: codons 1 – 376 UL30: codons 1 - 1240
Compatible extraction methods
Automatic (MagNA Pure Compact Nucleic Acid Isolation Kit I - Roche, Promega - Abbott), Manual (Manual extractions using QIAamp® Viral RNA - Qiagen)...
Data analysis and interpretation software
DeepChek-HSV
Available analyses
Amino-acid mutations detection, nucleotide changes detection, drug resistance, NGS-run quality report, clinical genotyping report...
Drug resistance
Aciclovir, Brivudin, Foscarnet, Penciclovir and Cidofovir
Included services
Unlimited updates & upgrades of the software, support, training...
Options
Local servers, historical data import, integration with LIS and HIS, integration with sequencers, automation of the IT workflow, customization...
EXAMPLES OF REPORTS
Ordering information
Product
Reference
DeepChek®Assay -HSV TK/POL (RUO)
119A24
