DeepChek®-CMV UL54/UL97 Assay

K-18-UL5497

Description

The DeepChek®-CMV UL54/UL97 Assay (K-18-UL5497) is intended to determine antiviral drug resistance to cidofovir, foscarnet sodium, ganciclovir and maribavir through PCR/sequencing (NGS).

Methodology

DNA Sequencing • Polymerase Chain Reaction (PCR)

More information on the DeepChek® Assays – Click here
More information on the DeepChek® Software – Click here

CHARACTERISTICS AND PERFORMANCES

Features

Compatibility with Next Generation Sequencing platforms

Validated on MiSeq, MiniSeq, (iSeq-100 ongoing) Ion PGM, Ion S5

Intended use

RUO – ISO-9001 manufacturing

Types of samples

Serum, plasma and whole blood

Format

24 samples/kit

Content

PCR reagents (enzymes, master mixes, primers, dNTPs...), protocols for NGS sequencing, NGS software analyses for 24 samples via a Cloud access

Sensitivity

1250 UI/mL for 1mL plasma/serum. Protocols for low viral loads (>200 - 300 UI/mL) available

Specificity

High

Reproducibility

>99%

Workflow

From sample to result in ~30 hours for NGS (depending on the platform)

Covered positions

UL54: codons 1 – 1243 UL97: codons 1 - 708

Compatible extraction methods

Automatic (MagNA Pure Compact Nucleic Acid Isolation Kit I - Roche, Promega - Abbott), Manual (Manual extractions using QIAamp® Viral RNA - Qiagen)...

Data analysis and interpretation software

DeepChek-CMV

Available analyses

Amino-acid mutations detection, nucleotide changes detection, drug resistance, NGS-run quality report, clinical genotyping report...

Drug resistance

Cidofovir, Foscarnet, Ganciclovir, Maribavir based on 2 different up-to-date guidelines

Included services

Unlimited updates & upgrades of the software, support, training...

Options

Local servers, historical data import, integration with LIS and HIS, integration with sequencers, automation of the IT workflow, customization...

EXAMPLES OF REPORTS

 

Ordering information

Product

Reference

DeepChek®-CMV UL54/UL97 Assay

K-18-UL5497