Description
The DeepChek®-HCV 5’UTR Genotyping Assay (RUO) is intended to be used for Viral Hepatitis C (HCV) genotyping and subtyping. It combines target-specific PCR reagents with in vitro diagnostic software both compatible with either Sanger or Next Generation Sequencing platforms.
Methodology
DNA Sequencing • Reverse Transcriptase Polymerase Chain Reaction (RT-PCR)
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Characteristics and performances
Features
Compatibility with Capillary Electrophoresis (SANGER) platforms
Any
Compatibility with Next Generation Sequencing platforms
Any
Intended use
RUO – ISO-9001 manufacturing
Types of samples
Serum, plasma, DBS
Format
24 samples/kit
Content
RT-PCR reagents (enzymes, master mixes, primers, dNTPs...), SANGER sequencing primers, protocols for SANGER and NGS sequencing, SANGER or NGS software analyses for 24 samples through a Cloud access
Sensitivity
1000 UI/mL for 400 µL plasma/serum. Protocols for low viral loads (>200 - 300 UI/mL) available
Specificity
Validated on all genotypes (pan-genotypic)
Reproducibility
>99%
Workflow
From sample to result in ~15 hours for SANGER and ~30 hours for NGS (depending on the platform)
Amplicon length
5'UTR: 244 bp
Compatible extraction methods
Automatic (MagNA Pure Compact Nucleic Acid Isolation Kit I - Roche, Promega - Abbott), Manual (Manual extractions using QIAamp® Viral RNA - Qiagen)...
Data analysis and interpretation software
Included (DeepChek-HCV / CE-IVD)
Available analyses
Subtyping, genotyping, NGS run quality report, clinical genotyping report...
Included services
Unlimited updates & upgrades of the software, support, training...
Options
Local servers, historical data import, integration with LIS and HIS, integration with sequencers, automation of the IT workflow, customization...
Examples of reports
For NGS sequencing
Ordering information
Product
Reference
DeepChek®-HCV 5’UTR Genotyping Assay (V2) (RUO)
110B24
DeepChek®-HCV 5’UTR Genotyping Assay (V3) (RUO)
110C24
Downloads
General documentation
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Protocol
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Installation check list
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Q&A
MSDS
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International