The DeepChek® Assay -HIV Assay Protease / Reverse Transcriptase Genotyping and Drug Resistance is intended to be used for HIV-1 genotyping and provides antiretroviral susceptibility information for protease inhibitors (PI) and reverse transcriptase inhibitors (NRTI, NNRTI). It combines target-specific PCR reagents with in vitro diagnostic software both compatible with either Sanger or Next Generation Sequencing platforms.
DNA Sequencing • Reverse Transcriptase Polymerase Chain Reaction (RT-PCR)
This test is not available for sale in the U.S. – CE marking only valid for EEA and territories recognizing it – Otherwise For Research Use Only (RUO). Not for use in diagnostic procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of disease. See product REF 101B24.
Characteristics and performances
Compatibility with Capillary Electrophoresis (SANGER) platforms
Compatibility with Next Generation Sequencing platforms
CE-IVD – ISO-9001 manufacturing
Types of samples
Serum, plasma, pro-viral DNA, DBS
RT-PCR & Nested-PCR reagents (enzymes, master mixes, primers, dNTPs...), SANGER sequencing primers, protocols for SANGER and NGS sequencing, SANGER or NGS software analyses for 24 samples through a Cloud access
1000 cp/mL for 400 µL plasma/serum. Protocols for low viral loads (>200 - 300 cp/mL) available
Validated on most of the subtypes and CRF strains
From sample to result in ~15 hours for SANGER and ~30 hours for NGS (depending on the platform)
PR: codons 1 to 99, RT: codons 1 to 320
Compatible extraction methods
Automatic (MagNA Pure Compact Nucleic Acid Isolation Kit I - Roche, Promega - Abbott), Manual (Manual extractions using QIAamp® Viral RNA - Qiagen)...
Data analysis and interpretation software
Included (DeepChek-HIV / CE-IVD)
Subtyping, amino-acid mutations detection, nucleotide changes detection, drug resistance, NGS run quality report, GSS, clinical genotyping report...
Flexible, through up to 7 different up-to-date guidelines including HIVdb/Stanford, ANRS, geno2pheno, Rega,...
Unlimited updates & upgrades of the software, support, training...
Local servers, historical data import, integration with LIS and HIS, integration with sequencers, automation of the IT workflow, customization...
Examples of reports
For SANGER sequencing
For NGS sequencing
DeepChek®Assay -HIV Assay Protease / Reverse Transcriptase Genotyping and Drug Resistance (CE-IVD)
Installation check list
EC Declaration of Conformity (121A24)